NuSep offers a large selection of nitrocellulose (NC) and polyvinylidene difluoride (PVDF) transfer membranes for immunoblotting applications. Choose from convenient pre-cut membranes sized for protein minigels and economical membrane rolls. All membranes are optimized for compatibility with a wide range of protein stains and enzyme-based detection systems, specifically with chemiluminescent substrates.
The PVDF membranes provide durability and high tensile strength for ease of handling. Proteins can be electroblotted from a variety of gel matrices onto PVDF-PLUS and will not pass through the membrane. It can be used for efficient transfer of proteins ranging in molecular weight from 5k to 700k daltons. The binding capacity of PVDF-PLUS averages 125µg/cm2 for larger globular proteins such as immunoglobulins with higher capacities for small peptides. PVDF-PLUS’s chemical compatibility allows its use with all common stains, such as Amido Black, Colloidal Gold, Coomassie Blue, India Ink, and Porceau-S. The membrane will not degrade, distort, or shrink when used with high concentrations of methanol for destaining.
The nitrocellulose membranes are pure nitrocellulose media that has high binding capacity for proteins. These nitrocellulose membranes are stronger and more durable than other nitrocellulose membranes, and will not rip, tear, or crack during transfers. The nature of this material yields sharper images with little or no distortion.
|DBM-NIM-02||Transfer Membrane NC 0.45µm (8cm x 8cm) Pk15|
|DBM-NIM-03||Transfer Membrane NC 0.2µm (8cm x 8cm) Pk15|
|DBM-NIM-04||Transfer Membrane NC 0.45µm (7.9cm x 10.5cm) Pk15|
|RB-500||Transfer Membrane NC 0.45µm (3cm x 8.5cm) Pk10|
|RB-600||Transfer Membrane NC 0.22µm (7cm x 8.4cm) Pk10|
|RB-700||Transfer Membrane NC 0.45µm (7cm x 8.4cm) Pk10|
|RB-800||Transfer Membrane PVDF 0.45µm (3cm x 8.5cm) Pk10|
|RB-900||Transfer Membrane PVDF 0.2µm (7cm x 8.4cm) Pk10|
|RB-1000||Transfer Membrane PVDF 0.45µm (7cm x 8.4cm) Pk10|