Frequently Asked Questions

Yes, we offer

  • 8-16% for mild-range separations
  • 4-20% for wide-range seperations

No, NuSep gels are compatible with most popular mini-gel tanks.

  • NN Cassette – 10cm x 10cm – fits XCell Sure Lock tanks (Novac)
  • NB Cassette – 10cm x 8.5 cm – fits Mini-Protean tanks (Biorad)
  • NG Cassette – 10cm x 8cm – is compatible with most generic tanks

All the gels have a 4% stacking gel.

No, the gels do not contain SDS (SDS is incorporated in the running buffer solution). The gels can be used for native runs.

NuSep gels and sample buffers do not contain reducing agents; they can be added to a sample prior to loading. Beta-mercaptoethanol and DTT are both suitable for use with our gels. 

*see Running Formulations

Yes, NuSep gels must be air-dried. Use of vacuum drying can result in cracking. NuSep gels can only be dried crack-free with NuSep Gel Drying Solution; all other drying solution can result in cracking. A recommended gel drying protocol can be found on the NuSep website.

Troubleshooting

Cause: Air bubbles in the sample wells, or between the gel and cassette, or at the bottom of the cassette

Solution: Use a transfer pipette to displace the air bubbles from the sample wells

Cause: Poorly soluble or weakly charged particles (such as carbohydrates) in the sample

Solution:

  1. Centrifuge sample
  2. Change pH of buffer
  3. Heat sample in the presence of SDS

Cause: Incorrect gel selection, sample overloading, insufficient cooling buffer

Solution:

  1. Reduce sample size
  2. Select a gel which separates in the desired molecular weight range
  3. For proteins of similar molecular weight, a 2-D separation may be required
  4. Increase buffer in outer tank

 

Cause: Too much salt in the sample

Solution: Reduce salt by dialysis or ultra-filtration

Solution: Enzymatically remove the carbohydrate

Solution: Use gel with large pore size at top, try addition of a non-ionic detergent

Cause: Excessive heating

Solution: Always start will chilled buffer (<15C)

Cause: SDS still present in gel

Solution: Fix in 10% TCA prior to staining, otherwise use 30% methanol in the destaining soution

COAs

COA Request Form
ex. NB10-008

For questions, email us at support@nusep.com.

NN Gels 10-well

181114      180607      180328

181114      180607      180206 

180920      180605      180410

181019      181016      181009     180822
180717      180704      180522

NN Gels 12-well

181122      181025      180828      190118

181113      181019      181009      180828

NN Gels 17-well

180124      181205      181218     

180124      181205      181218      181219

NB Gels 10-well

NB Gels 12-well

180816      180725      180425      180111

180724      180802      180926

180110      180724      180726      180814
180918

NB Gels 17-well

NG Gels 10-well

180123      180408      180821

180403      180615     190115

180123      180517      180626

180428      180601      180615      180705
180912      181017

NG Gels 12-well

180109      180322      180710

180117      180321      180411      180428
180504      180516      180525      180601
180614      180627      180831      180906
181012

NG Gels 15-well

181023      181010      180907      180622
180530      180515      180503      180426
180425      180320      180316      180314