Blocking Buffer

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Blocking Buffer

Blocking Buffer

BlockPRO Blocking Buffer

BlockPRO Blocking Buffer is suitable for blocking in Western blot, ELISA, immunohistochemistry and other immunochemical application. It can block the excess binding site but not cover the binded protein of the membrane and therefore increase the signal intensity, presenting better data result than milk and BSA blocking buffer.

Special Characteristics
High efficiency : Increase signal intensity
High compatibility : Suitable for downstream PBST and TBST wash procedure and also compatible with every immunochemical application
Simple : Safe and ready to use

Performance

BlockPRO Blocking Buffer - AMPK compared with Casein, BSA, and Skim Milk

Figure 1. Signal strength comparison of Casein, BSA, milk, and BlockPRO™ Blocking Buffer. Loading 30 μg cell lysate (HepaG2) and detect with anti-AMPK (mouse, 1:1,000). Secondary antibody : anti-mouse IgG-HRP 1:10,000. Membrane: Hyond™ P. Detection : Hyperfilm™ ECL. All results were exposed for 30 seconds and capture by X-ray film.

BlockPRO Blocking Buffer v Skim Milk

Figure 2. Blocking with skim milk or BlockPRO™ Blocking Buffer and detect by SEM. 30 μg cell lysate (HepaG2) separated by 12.5% SDS-PAGE and blocking by skim milk or BlockPRO™ Blocking Buffer. Membranes detected by SEM. The SEM result showed that BlockPRO™ Blocking Buffer only blocks on excess binding site and reveals the position of antigen, therefore it will not reduce the signal intensity.

Downloads
MSDS_BlockPRO blocking buffer
Manual_BlockPRO blocking buffer

BlockPRO Protein-Free Blocking Buffer is a non-protein formulation that enhances sensitivity and minimizes background noise, presenting better results than traditional protein-based blocking buffer in immunoassays. The synthetic formulation of BlockPRO Protein-Free Blocking Buffer makes it suitable for PVDF and NC platform, avidin/biotin system, detection of phosphoprotein, and other immunochemical applications.

Special Characteristics

High performance: Provide better specific signal and less background noise than traditional blocking buffer
Multiple application: Suitable for Western blot, dot blot, ELISA, and other immunoassays
High quality control: Ensure lot-to-lot consistency for your most reproducible results over time

Performance

BlockPRO Protein-Free Blocking Buffer - THP-1 Cell Lysate compared with Skim Milk, BSA, and Casein

Figure 3. BlockPRO™ Protein-Free Blocking Buffer is better than protein-based blocking buffers (skim milk, BSA and casein) for detection of target protein in Western blotting. THP-1 cell lysates were prepared and separated by electrophoresis. The proteins were transferred to PVDF and blocked for 1 hour at room temperature with the indicated blocking buffer, probed with mouse anti-pAMPK followed by anti-mouse HRP and detected by chemiluminescence. All results were exposed to X-ray film for 30 seconds.

Figure 4. BlockPRO™ Protein-Free is suitable for various protein antigen detection, such as high molecular weight protein, tACC; phosphoprotein, pAMPK; abundant protein, GAPDH; low molecular weight protein, histone H3. THP-1 cell lysates were prepared and separated by electrophoresis. The proteins were transferred to PVDF and blocked for 1 hour at room temperature with BlockPRO™ Protein-Free Blocking Buffer. Antibodies designed to probe the indicated proteins were used. All the signals were detected by chemiluminescence and were exposed to X-ray film.

Figure 5. BlockPRO™ Protein-Free Blocking Buffer can be used in both PVDF and nitrocellulose platform. Hela cell lysates were prepared and separated by SDS-PAGE. The proteins were transferred to PVDF or nitrocellulose membranes. The membranes were blocked for overnight at 4 °C with BlockPRO™ Protein-Free Blocking Buffer or 5% skim milk, probed with mouse anti-histone H3 followed by anti-mouse HRP and detected by chemiluminescence. All results were exposed to X-ray film for 30 seconds.

Downloads
MSDS BlockPRO Protein-Free Blocking Buffer
Manual BlockPRO Protein-Free Blocking Buffer

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