NuSep PVDF Membranes are ideal for chemiluminescent and colorimetric western blots: it retains target protein very strongly during protein transfer but also reduces nonspecific protein binding that can obscure high-sensitivity detection.

Features and Benefits

• Mechanical strength of PVDF membranes enable multiple reprobing of blots
• Naturally hydrophobic support is ideal for western blotting because it ensures high signal with low background
• Available in three formats:
  • Convenient precut membrane/blot absorbent filter paper sandwiches
  • Packs of precut membrane-only sheets
  • Economical rolls

Applications

• Transferring proteins from SDS-PAGE gels to membrane
• Immunoblotting target proteins
• Detection by western blotting chemiluminescent or colorimetric methods

Western Blotting Protocol