nUView significantly improves SDS-PAGE procedures for validating protein purity. Because chromatography is not effective enough on its own to ensure protein purity for critical applications such as drug discovery, effective orthogonal procedures are necessary. nUView technology drastically reduces run times while increasing sensitivity, dynamic range, and the lower limit of detection. When coupled with advances to automated chromatography systems, protein purification workflows can be completed faster and at lower cost than ever before at both small and process scale.