NuSep Team

👨‍🔬 Check it out: Researchers at the  [AxioMx, Abbratech Inc, Branford, CT, USA.] have developed a surrogate protein (‘MILKSHAKE’) using modified maltose binding protein (modMBP) and a target protein peptide containing a modified or nonmodified residue of interest for use in Western blot.

Read the full article here:https://www.future-science.com/doi/10.2144/btn-2021-0078

Credit: Jones, K. S., Chapman, et al. Milkshake: Novel validation method for antibodies to post-translationally modified targets by surrogate western blot. BioTechniques, 72(1), 11–20, (2022). https://doi.org/10.2144/btn-2021-0078 

Sortase-mediated conjugation
Figure 1. Sortase-mediated conjugation of modified maltose binding protein with target peptides