NuSep offers empty 1ml, 5ml, 10ml, and 20ml FPLC chromatography columns. FPLC columns meet the demands of research in both academia and biopharma. Both ends of the FPLC column have 10-32 UNF threads which fits all common chromatography instruments, such as AKTA.

Pack your own resin into these columns. Separate your proteins using an FPLC, HPLC, low pressure pump or syringe. These easy to use columns are user friendly, robust and suitable for a range of applications.

Product Technical Information:

Maximum pressure 1 ml column: 5 bar (70 psi)
Maximum pressure 5, 10 & 20 ml column: 3 bar (42 psi)
pH stability: 2-14
Recommended flow rate: 0.5-2 ml/min
Chemical compatibility: All common aqueous solutions
Denaturants: 8 M Urea, 4 M guanidine hydrochloride and chaotropic salts.
1 ml column housing: Medical grade polypropylene
5 ml, 10 ml & 20 ml column housing: Acrylic
Bed dimensions of the 1 ml column: 33 x 6.2 mm i.d.
Bed dimensions of the 5 ml column: 52 x 11.0 mm i.d.
Bed dimensions of the 10 ml column: 104 x 11.0 mm i.d.
Bed dimensions of the 20 ml column: 100 x 16.0 mm i.d.
End Adaptors (polypropylene): 10-32 UNF female thread (1/16″)

Proteus FliQ FPLC Column